A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
In recent years, plant virus-based vectors have been widely applied to express heterologous proteins for genomic studies and commercial production. Among these versatile RNA viral vectors, the barley yellow striate mosaic virus (BYSMV)-based expression vector system has outstanding capability to express large and multiple heterologous proteins. Here we describe a detailed protocol for expression of heterologous proteins using BYSMV expression systems in monocot plants and insects.
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关键词
Barley yellow striate mosaic virus,Monocot plants,RNA viral vectors
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