[Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn].

PURPOSE:To investigate whether Ti-35Nb-2Ta-3Zr/Zn (TNTZ/Zn) composite material processed by friction stir processing has osteogenic activity. METHODS:Zn was added to the surface of TNTZ alloy by friction stir processing. The control group was TNTZ(TNTZ without FSP), the experimental groups was FSP(FSP without Zn), TNTZ/Zn-0.5 (0.5 mm pre-made holes) and TNTZ/Zn-1(1 mm pre-made holes). Surface characterization was performed by scanning electron microscope and X-ray diffraction. Alkaline phosphatase(ALP) activity of co-cultured rat bone marrow stromal stem cells (BMSCs) and COL-1α, OPN and OCN gene expression were detected by real-time quantitative PCR. SAS version 8.2 software was used for data analysis. RESULTS:Zn nanoparticles on the surface of the TNTZ/Zn-1 group were distributed homogeneously, with a diameter range of 70-80 nm. Compared with TNTZ group, the activity of ALP in TNTZ/Zn-0.5 and TNTZ/Zn-1 groups was up-regulated (P<0.05 and P<0.01). Compared with TNTZ group, ALP, COL-1α, OPN and OCN gene expression increased in TNTZ/Zn-0.5 and TNTZ/Zn-1 group, and the difference was statistically significant (P<0.05). CONCLUSIONS:Through FSP, Zn can be successfully integrated onto the surface of TNTZ alloy with nano-scale microstructures. TNTZ/Zn composite material can effectively induce bone formation and is a potential implant material.