Experimental design of a stable isotope labeling derivatized UHPLC–MS/MS method for the detection/quantification of primary/secondary bile acids in biofluids

Yoshio Muguruma,Ryosuke Nagatomo,Shihori Kamatsuki,Katsuyuki Miyabe,Go Asano,Hiroyasu Akatsu,Koichi Inoue

Journal of Pharmaceutical and Biomedical Analysis（2022）

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摘要

•Experimental design was applied to achieve the optimal derivatization of bile acids.•Stable isotope labeling method was validated for bile acids profiling by UHPLC–MS/MS.•Derivatized UHPLC–MS/MS was utilized to profile 11 bile acids in human biofluids.•Correlation between the bile acids in serum and bile was investigated using paired samples.

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关键词

Design of experiments,Bile acids,Short-chain fatty acids,Serum,Bile,Stable isotope labeling derivatization,Ultrahigh performance liquid chromatography–tandem mass spectrometry

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