Lignin removal, reducing sugar yield and photo-fermentative biohydrogen production capability of corn stover: Effects of different pretreatments.

The effects of different pretreatment methods, including hydrothermal, acid, alkali, acid-heat and alkali-heat on lignin removal, reducing sugar (RS) yield and photo-fermentative biohydrogen production (PFHP) capability of corn stover (CS) were studied. NaOH-heat pretreatment was the most effective for lignin removal from CS, and the lignin removal rate reached 77%. All the studied pretreatment methods improved the total RS yield of CS, and the highest total RS yield (46.1 g/100 g raw material (RM)) was obtained from 2% NaOH-heat pretreated CS. 2% NaOH pretreatment realized the best PFHP of CS, which increased the hydrogen yield (HY), maximal hydrogen production rate (HPR) and highest hydrogen content (HC) by 31.9%, 50.9% and 20.1% respectively, and shortened hydrogen production lag time (HPLT) by 58.8% over that of untreated CS. However, NaOH-heat and 4% NaOH pretreatment weakened the PFHP capability of CS.