Deterministic processes drive the microbial assembly during the recovery of an anaerobic digester after a severe ammonia shock

Anaerobic digestion allows to produce sustainable energy but the microbial community involved in this process is highly sensitive to perturbations. In this study, a longitudinal experiment was performed in two sets of trip-licate bioreactors to evaluate the influence of ammonia addition on AD microbiome and its recovery. Zeolite was added in three reactors to mitigate the inhibition. Microbial dynamics were monitored with 16S rRNA sequencing at 15 time points. Dominant methanogenic pathways were determined with gas isotopic signature analysis. Zeolite addition did not enable to reduce ammonia inhibition or improve the process under the con-ditions tested. In all the bioreactors, ammonia inhibition sharply decreased the methane production but the process could restart thanks to the increase of hydrogenotrophic archaea and syntrophic bacteria. Interestingly, similar behaviour was observed in the six reactors. Neutral modelling and null model were used and showed that a deterministic process governed the recovery of AD microbiome after failure.