Fe²⁺ Alleviated the Toxicity of ZnO Nanoparticles to Pseudomonas tolaasii Y-11 by Changing Nanoparticles Behavior in Solution

The negative effect of ZnO nanoparticles (ZnO-NPs) on the biological removal of nitrate (NO3-) has received extensive attention, but the underlying mechanism is controversial. Additionally, there is no research on Fe2+ used to alleviate the cytotoxicity of NPs. In this paper, the effects of different doses of ZnO-NPs on the growth and NO3- removal of Pseudomonas tolaasii Y-11 were studied with or without Fe2+. The results showed that ZnO-NPs had a dose-dependent inhibition on the growth and NO3- removal of Pseudomonas tolaasii Y-11 and achieved cytotoxic effects through both the NPs themselves and the released Zn2+. The addition of Fe2+ changed the behavior of ZnO-NPs in an aqueous solution (inhibiting the release of toxic Zn2+ and promoting the aggregation of ZnO-NPs), thereby alleviating the poisonous effect of ZnO-NPs on the growth and nitrogen removal of P. tolaasii Y-11. This study provides a theoretical method for exploring the mitigation of the acute toxicity of ZnO-NPs to denitrifying microorganisms.