A glycosylphosphatidylinositol-anchored α-amylase encoded by amyD contributes to a decrease in the molecular mass of cell wall α-1,3-glucan in Aspergillus nidulans
Frontiers in Fungal Biology(2021)
Abstract
α-1,3-Glucan is one of the main polysaccharides in the cell wall of Aspergillus nidulans . We previously revealed that it plays a role in hyphal aggregation in liquid culture, and that its molecular mass (MM) in an agsA -overexpressing ( agsAOE ) strain was larger than that in an agsB -overexpressing ( agsBOE ) strain. The mechanism that regulates the MM of α-1,3-glucan is poorly understood. Although the gene amyD , which encodes glycosyl-phosphatidylinositol (GPI)-anchored α-amylase (AmyD), is involved in the biosynthesis of α-1,3-glucan in A. nidulans , how it regulates this biosynthesis remains unclear. Here we constructed strains with disrupted amyD (Δ amyD ) or overexpressed amyD ( amyDOE ) in the genetic background of the ABPU1 (wild-type), agsAOE , or agsBOE strain, and characterized the chemical structure of α-1,3-glucans in the cell wall of each strain, focusing on their MM. The MM of α-1,3-glucan from the agsBOE amyDOE strain was smaller than that in the parental agsBOE strain. In addition, the MM of α-1,3-glucan from the agsAOE Δ amyD strain was greater than that in the agsAOE strain. These results suggest that AmyD is involved in decreasing the MM of α-1,3-glucan. We also found that the C-terminal GPI-anchoring region is important for these functions.
### Competing Interest Statement
The authors have declared no competing interest.
MoreTranslated text
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined