Preparation Of Gamma-Polyglutamic Acid From Enzymatic Hydrolysate Of Poplar Sawdust
ARABIAN JOURNAL OF CHEMISTRY(2021)
摘要
The treatment of forestry waste has always been an environmental problem. In this study, poplar sawdust was used to produce gamma-polyglutamic acid (gamma-PGA). Poplar sawdust was treated with acid and then hydrolyzed with cellulose. The enzymatic hydrolysate was used to produce gamma-PGA. Through single factor and orthogonal experiments, the optimum enzymatic conditions were determined as follows: solid-liquid ratio 1:5, enzyme amount 22 FPU/g, pH 5.0 and 53 min. Under the enzymatic hydrolysate medium, the gamma-PGA yield reached 30.87 +/- 0.44 g/L, which was 5.11% higher than that in glucose medium (29.37 +/- 0.43 g/L). In addition, the amount of glucose added to the medium was reduced, which realized the comprehensive utilization of various sugars in sawdust enzymatic hydrolysate. Although the fermentation time was prolonged by 4 h, this study provided a new and feasible method for the industrial utilization of forestry waste and the clean production of gamma-PGA. (C) 2021 The Author(s). Published by Elsevier B.V.
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关键词
Poplar sawdust, Bacillus subtilis, gamma-polyglutamic acid
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