Clonal Fidelity Assessment Of In Vitro Propagated Bambusa Balcooa Plant Using Dna Marker

Efficient in vitro micropropagation protocol of Bambusa balcooa was established. Nodal segment was used as explant for propagation. Out of the various sterilization treatments tested, S7 treatment supplemented with PPM in culture medium shown minimum contamination percentage i.e. 13.33%. Culture initiation in solid Murashige and Skoog (MS) media supplemented with BAP (1,1.5,2,3,4,5,6 mg/l).4 BAP was best for culture initiation with 90% response. Cultures were multiplied using MS medium supplemented with different hormones. M7 treatment containing of Kn (2mg/l) and BAP (4 mg/l) gave best response with +/- 6.6 +/- 0.24 multiple shoots per explants. Root induction was done in MS medium supplemented with NAA (5 mg/l) has induced 6.0 roots per explant.Rooted plantlets were successfully acclimatized with soil and coco-peat (1:1; v/v) under primary hardening condition in green house (1 month). After primary hardening, plants were transferred to the polybag for secondary hardening in sand, soil, cocopeat (1: 1: 1; v/v). Clonal fidelity test for these randomly selected micro propagated plants was done using 14 ISSR (Inter Simple Sequence Repeat) markers, out of which 8 markers were amplified within range of 320-950 bp bands. The result has shown complete uniformity among randomly selected micro propagated plants.