An Enhanced Extraction And Enrichment Phytochemicals From Rosa Roxburghii Tratt Leaves With Ultrasound-Assist Co2-Based Switchable-Solvent And Extraction Mechanism Study

An efficient and ecofriendly extraction and enrichment process from Rosa roxburghii Tratt leaves using a CO2-responsive switchable solvent with ultrasound assistance based on a N,N-dimethyl-cyclohexylamine (DMCHA)/water system was developed in this study. We applied single-factor experiments combined with a Box-Behnken design to obtain the best operation conditions. The optimal parameters were as follows: bubbling CO2 rate of 40 mL/min, bubbling CO2 time of 29 min, and ultrasound time of 11.5 min. The yields of representative roxburic acid, kajiichigoside F1, pomonic acid, catechin, epicatechin, kaempferol, rutin, and quercetin reached 1.31 +/- 0.02, 1.89 +/- 0.01, 1.62 +/- 0.02, 0.56 +/- 0.015, 1.24 +/- 0.024, 0.074 +/- 0.002, 0.13 +/- 0.001, and 1.56 +/- 0.028 mg/g, respectively. The eight phytochemical extraction efficiencies and contents increased observably compared with traditional extraction methods. This process could achieve 5 cycles of extraction without obvious loss. Moreover, the properties of SHS and their relationship with the eight compounds were investigated by density functional theory. This process is a promising alternative for industrial manufacturing. (C) 2021 Elsevier B.V. All rights reserved.