The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

Objective:To develop colloidal gold immunochromatographic(IC) method for rapidly detecting the concentration of Sodium Cholate.Methods: Sodium Cholate-BSA immunogen was obtained via carbodiimide method.Then the immunoantigen was injected into New Zealand rabbits to prepare the anti-sodium cholate polyclona antibody according to routine immunity procedure.The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay(ELISA).Results: The sodium cholate-OVA antigen-colloidal gold compounds were made for test strip preparation.It was proved that the specificity of the antibody was 1 ∶ 80 000,and the detectability was 12 μg/mL by the method.Conclusion: Accordingly,IC test strip method is effective and sensitive for rapidly detecting sodium cholate,which will probably have a broad clinical application prospect.