Determination of some elements in the nails in patients with colon cancer using total reflection X-ray fluorescence

Sixty-two subjects (31 men with colon cancer) participated in this study. The analysis of elements in the nails was performed by total reflection X-ray fluorescence (TXRF) technique. The results showed that fifteen elements were quantified, including the following: S, P, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, and Br. We have found that there were significant differences in the concentrations of elements Ca, Cr, Fe, Cu, Zn, As, and Se between normal subjects and men with colon cancer (p < 0.05). We conclude that TXRF is a technique suitable for elemental analysis in human nail samples. The concentration of elements Ca, Cr, Fe, Cu, Zn, As, and Se could be among the factors used for the diagnosis of colon cancer risk in men.