Phytochemical Fabrication Of Silver Nanoparticles Using Camellia Sinensis To Probe Their Biological Efficacy

One of the most sought-after practices that determine the success of tiny technology relies on broad spectrum applications and environmental safety. A simple one pot method was adopted to fabricate stable silver nanoparticles using Camellia sinensis leaf extract. The synergistic antimicrobial effect of AgNPs was evaluated upon amalgamation with PLGA nanofibre. Characterization studies were performed using UV visspectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The antibacterial effect of the synthesized NPs immobilized PLGA nanofibre (PAN) was evaluated using agar diffusion method. Nanoparticle synthesis could be achieved rapidly within 30 minutes of the reaction demonstrating a single absorbance peak at 421 nm (lambda(max)) XRD and SEM results showed that particles were crystalline and circular in shape with size < 40nm. Phytochemical reduction and capping of NPs could clearly be evidencedfrom FTIR studies.Antimicrobial susceptibility screening ofAgNPs (5, 10, 20, 40 mu g) performed using clinical isolates (n = 6) showed significant growth inhibition at higher concentrations. Similarly, nanoparticulate efficacy of AgNPs-PLGA membrane in overcoming resistance was demonstrated using their corresponding resistant counterparts. They forecasted significant inhibition in growth against all the resistant strains (individual and consortia) at 30 mu g concentration at par with conventional antibiotics at a given concentration. Such prepared nanoparticles inducing potential antibacterial activity might be fine-tuned prior to surface modification and biocompatibility studies for broad spectrum therapeutic modalities.