Effect of Methanol and Mineral Nitrogen Compounds on the Composition of Methanotrophic Enrichments from the Sediments of a Lake Baikal Methane Seep

Diversity of methano- and methylotrophic bacteria in enrichments from the oxidized sediment layer maintained at 10°C in mineral media with nitrogen compounds ( NO_3^ - and NH_4^ + ) and methanol was studied using high-throughput sequencing of the V2–V3 region of the 16S rRNA gene. Methanotrophs of the bottom sediment belonged to the order Methylococcales , with predominance of the genus Methylobacter (6.4 and 4.6