Antidyslipidemic And Anti-Oxidant Activities Of Nigella Sativa Seeds Extract In Hyperlipidemic Rats

This experimental study had approved by animal ethics of Central Drug Research Institute, Lucknow, and had undertaken to evaluate the antidyslipidemic and anti-oxidant activities of Nigella Sativa (N. Saliva), Hindi name Kalonji seeds extract in two models of hyperlipidemia. 1- triton and 2-cholesterol-rich high-fat diet (HFD) induced hvperlipidemia. N. Sativa and Gemfibrogil were macerated with 2% aqueous gum acacia, and the suspension had fed orally to rats at a dose of 500 mg/Kg (b.w.p.o.) respectively. Serum lipids were found to be lowered by N. Saliva in triton induced hvperlipidemia. On the other hand chronic feeding of N. Saliva extracts to rats in cholesterol-rich high fat diet-induced hvperlipidemia for 30 days caused lowering in lipid and apoprotein levels of beta-lipoproteins followed by an increase in lipid and apoprotein levels of alpha lipoproteins. The results of the present study also demonstrate that N. Saliva seeds extract repaired hepatic lipid synthesis, increased fecal bile acid excretion, and increased plasma LCAT activity in rats. Furthermore, N. Sativa seeds extract (100 to 400 mu g/ml) inhibited the in vitro generation of superoxide anions and hydroxyl radicals in both enzymatic and non-enzymatic systems in a concentration-dependent manner.