In Vitro Anti-Diabetic And Anti-Inflammatory Activities Of Metabolites Isolated From Marine Sponge, Heteronema Erecta (Keller, 1889) And Its In Silico Studies

Phenylalanine, aromatic amino acid and clupadonic acid, a long-chain fatty acid were purified from methanol and methanol-dichloromethane extracts of marine sponge H. erecta Keller. It was characterized by using UHPLC- Triple quadrupole ESI/MS. The clupadonic acid isolated possessed good inhibition towards alpha-amylase and alpha-glucosidase with IC50 of 17 mu g/ml and 22 mu g/ml respectively. Apart from that, phenylalanine gave better anti-inflammatory compared to clupadonic acid with 16 mu g/ml. The interaction between phenylalanine and clupadonic acid against the three receptors IXU7, 1SMD and 4Y14 has been studied using molecular docking.