Biological Functions Of Mirna-188-5p/Xrcc5 In Glioma

Purpose: The purpose of this study was to uncover the influ- ence of microRNA-188-5p (miRNA-188-5p) on the malignant progression of glioma, thus providing a new direction in the early diagnosis and prediction of disease progression.Methods: MiRNA-188-5p levels in 44 glioma tissues and paracancerous ones were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Its influence on path- ological indicators and prognosis in glioma patients was analyzed. In glioma cell lines, regulatory effects of miRNA188-5p on cell phenotypes were examined by cell counting kit-8 (CCK-8), wound healing and Transwell assay, respectively. Moreover, the interaction between miRNA-188-5p and XRCC5, as well as their involvement in the development of glioma were finally illustrated.Results: MiRNA-188-5p was downregulated in glioma samples. Glioma patients expressing a low level of miRNA188-5p had a worse prognosis. Overexpression of miRNA188-5p remarkably attenuated proliferative and migratory abilities of glioma cells. XRCC5 was the downstream gene of miRNA-188-5p, and its level was negatively regulated by miRNA-188-5p. Besides, XRCC5 was upregulated in glioma samples. Moreover, XRCC5 was responsible for the inhibitory effects of miRNA-188-5p on the malignant progression of glioma.Conclusions: MiRNA-188-5p is linked to malignant progression in glioma patients, and it inhibits the proliferative and migratory abilities of glioma cells by binding XRCC5 and then negatively regulating its level.