Insight Into Anti-Diabetic Potential Of Clematis Apiifolia : Glucose Control In Diabetes

Antidiabetic activity of the methanolic extract of Clematis apiifolia was investigated in this research study. Methanolic, ethanolic and aqueous extracts were prepared and evaluated for three of the major (alpha-glucosidase, Antiglycation and Protein Tyrosine Phosphatase 1B) inhibitory assays. Cytotoxicity and Glucose consumption were studied in L6 cell line followed by PCR and Western blotting. Chemically induced diabetic mice model was used for in vivo antidiabetic activity. All the three extracts have shown significant enzymatic inhibitory activities while methanolic extract proved to be more compelling. Methanolic extract has shown no cytotoxicity in L6 cell line and promoted glucose consumption, with the highest glucose uptake at 1mg/mL, after treatment for 12 hours. These treatment conditions (1mg/mL, incubation time 12 hours) resulted in up regulation of mRNA level of GLUT4 while PTP1B get downregulated. Protein expression studied in western blot has given the results in line with results of PCR. In vivo study has shown the hypoglycemic effect of methanolic extract in diabetic mice comparable to standard drug glibenclamide.