Fasl-844t/C Mutation May Participate In Occurrence And Development Of Pulmonary Squamous Cell Carcinoma In South China

Fas-FasL system plays an important role in cancer initiation, development and progression. FasL -844T/C mutation has been shown to be significant in the genetic susceptibility to cancer. We explored the relationship between FasL gene -844T/C SNP and pulmonary squamous cell carcinoma (SCC). 330 patients with pulmonary SCC in south China were recruited from July 2007 to Oct 2011 in Zhejiang Cancer Hospital and their clinicopathological data were collected. 297 cases of cancer-free individuals were selected as control group. PCR-RFLP technique was carried out to detect FasL -844T/C single nucleotide polymorphism (SNP). Fas -1377G/A SNP was also detected to investigate whether it interfered with the functional effect of the FasL -844T/C in pulmonary SCC development. chi(2) test and logistic regression were used to analysis the association between FasL -844T/C, Fas -1377G/A polymorphism or other clinicopathological parameters and pulmonary SCC. Gender, smoking and FasL -844 genotypes could increase risk of pulmonary SCC susceptibility mainly in age <60 group. However, only smoking was associated with pulmonary SCC in age <60 group. Compared with TT genotype, FasL -844CC was risk factor for development of pulmonary SCC (adjusted OR=1.518, 95% CI=1.100-2.094, P=0.011). After grouping patients with smoking pack-years, it was a risk factor for pulmonary SCC in >= 20 pack-years group. There was no effect modification between FasL -844 SNP and smoking or gender by test of the interaction term (P<0.05). FasL -844C/T was significant associated with stage, lymph node metastasis and vascular tumor thrombus in age >= 60. Our results show that FasL -844T/C SNP is associated with the occurrence of pulmonary SCC in heavy smoking and younger people, and the development and metastasis in elder people.