Effects Of Scagp On The Biology Behavior Of Gastric Cancer Mgc-803 Cell

The current study demonstrated the effects of Sodium carboxyl-amino-glucan-polysaccharide (SCAGP) on the biology behavior of gastric carcinoma MGC-803 cells. In vivo, the mice inoculated intraperitoneally with gastric cancer MGC-803 cells were divided into control and treatment group (n = 20), which were intraperitoneally injected with saline (10 ml/kg body weight) and SCAGP (10 ml/kg body weight) daily for 10 days, respectively. After the mice were euthanized, the size and number of intraperitoneal cancer nodule were detected by laparotomy. In vitro studies, cell proliferation and survival assay were performed using the Cell Counting Kit-8 (CCK-8). Both of cell cycle distribution and cell apoptosis were identified by Flow Cytometry. Cell migration was detected by Transwell assay. The phosphorylation levels of several proteins involved in Akt and MAPK signal pathway were assessed by western blot analysis. Our data showed that in vivo SCAGP could markedly inhibit peritoneal metastasis of gastric cancer cells. In vitro, compared with the control group, SCAGP inhibited cell growth and proliferation, induced apoptosis and caused cell cycle arrest in the G0/G1 phase. The capacity of cellular migration was reduced significantly (P < 0.05). In addition, after treatment with SCAGP, the phosphorylation of AKT, extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun N-terminal kinases (JNK) were down-regulated, with the phosphorylation of p38 mitogen-activated protein kinase up-regulated in a dose-dependent manner. This study suggested that SCAGP may serve as a new kind of anti-tumor drug for gastric carcinoma treatment in the future.