Development Of A Triplex Real-Time Pcr System For The Differentiation Between Brassica Plant Species

Reliable monitoring of the authenticity of food and feed depends on correct analytical methods. One source of error might be false positive results. This might be especially the case with closely related or interrelated species. One such genus is Brassica which consists of the three diploid species Brassica rapa (A genome), B. nigra (B genome), and B. oleracea (C genome) and the three allopolyploid plant species B. juncea (AB), B. napus (AC), and B. carinata (BC). All six Brassica plant species have in common that they are used for food and feed worldwide. To be able to discriminate between all six of them in one real-time PCR run, a triplex real-time PCR system was established which identifies the three genomes (A, B, or C). The efficiency, R2, and LOD values are within the expected range. The applicability of monitoring the authenticity of processed food was shown by analyzing emulsified sausages with 0.0005-0.1% (w/w) rapeseed protein, 2% broccoli, or 0.1% brown mustard seeds. The detection and differentiation were possible at all concentration levels.