1340P The characteristics of the intestinal flora of advanced non-small cell lung cancer in China and its role in chemotherapy based on metagenomics

NSCLC is the most common type of lung cancer. Current studies have found that the role of gut microbes in tumor treatment is becoming more and more obvious. Adjusting the intestinal flora may affect the response of many forms of tumor treatment. 45 patients with advanced NSCLC who received platinum-containing dual-drug chemotherapy were enrolled. The intestinal flora was dynamically collected at baseline and after 2 cycles of chemotherapy. We use NGS and metagenomics to analyze the species and function of intestinal flora at all levels. At the family level, the abundances of Streptococcaceae, Lactobacillaceae, and Leuconostocaceae after platinum-containing dual-drug chemotherapy were significantly increased compared with those before chemotherapy. At the family level, the abundance of Leuconostocaceae in patients with gastrointestinal reactions caused by chemotherapy was significantly higher than that in patients without gastrointestinal reactions. At the same time, the KEGG database was used to find that the abundance of Metabolism, Human Diseases, Cellular Processes, and Environmental Information Processing is higher in patients with gastrointestinal reactions. At the species level, the abundances of Bacteroides coprocola, Bacteroides intestinalis, Bacteroides fluxus, and uncultured Bacteroides sp. in the response group were higher than those in non-responding patients, and these different intestinal flora belong to the genus Bacteroides. In addition, the abundance of the six major metabolic pathways in non-responding patients was higher than that in the response group. The intestinal flora of NSCLC patients is significantly different before and after chemotherapy, and it is related to the gastrointestinal reaction caused by chemotherapy and the efficacy of chemotherapy.