Protective Effect Of Costus Speciosus Rhizome Extract Against Cytotoxicity Induced By Tramadol In Mice

Tramadol is a centrally acting analgesic generally used to treat moderate to severe pain. This study as designed to evaluate the protective effect of Cosfus speciosys rhizome extract (CSRE) against the chromosomal 'aberration's tramadol-induced in mice bone marrow. Sixty male albino mice were examined in this study and separated into four groups 'which are as follcm s: Control group: CS RE group (which have been supplied with 200 mg/kg per day): Tramadol group (Supplied with 40 mg/kg): and CSRE Tramadol group ( which were given Tramadol for a period of 4 ecks and then given CS RE for a further 4 weeks. applying the same dosage. Microscopic examination of the bone marrow shoved a significant increase in structural chromosomal aberrations (centromeric attenuation. gap chromosome. ring chromosome. end to end association, and centric fusion) in addition to numerical chromosomal aberrations (polyploidy and endomitosis) in the treated mice with Tramadol comparable to mice in the control group. The administration of Tramadol disrupted oxidants -antioxidants balance. which is proven by the increased malondialdehydc (MDA) and the reduced superoxide dismutase (SOD). catalase (CAT). and glutathione (GSH). Interestingly, in contrast. CS RE administration has shown attenuate in the numerical chromosomal aberrations and biochemical modification caused by Tramadol. This study demonstrated that CSRE supplementation is improved the tramadol-induced cytotoxicity injury through its antioxidant activity