Sfrp2 Dna Hypermethylation And Decreased Protein Expression Of Escc In Kazakh And Han Patients

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in China, particularly in northwest area. However, no effective biomarkers have been identified for the diagnosis of patients with ESCC. It has been reported that Wnt signaling pathway plays an important role in Esophageal Cancer. Secreted frizzled-related protein 2 (SFRP2) is a further Wnt inhibitor whose expression was recently found being down-regulated in various malignancies, and its underlying mechanism are poorly understood. Here, our aim of the study was to investigate the expression levels and methylation status of the SFRP2 in ESCC, and to evaluate the clinical utility of the marker. Methods: In this study, we used immunohistochemistry (ICH) to measure the expression levels of SFRP2 in ESCC tumor tissue and the corresponding matched adjacent normal tissue. Methylation-special polymerase chain reaction (MSP) was employed to evaluate the aberrant methylation status of SFRP2 in DNA. Then, the correlation between SFRP2 protein expression and SFRP2 promoter methylation with clinicopathological parameters multiethnic differences and prognosis outcomes of ESCC patients were statistically analyzed respectively. Results: SFRP2 protein expression increased progressively from normal esophageal epithelium to ESCC. SFRP2 promoter methylation was detected in ESCC tumor tissues, whereas, a little normal tissues were affected by SFRP2 methylation. Of ESCC tumor tissues, most patients' tissues lacked SFRP2 protein expression due to SFRP2 promoter methylation. Conclusion: The present analysis demonstrated that SFRP2 may play an important role in the carcinogenesis and progression in terms of promoter methylation and protein expression.