The Influence Of Uv Femtosecond Laser Pulses On Bacterial Dna Structure, As Proved By Fourier Transform Infrared (Ft-Ir) Spectroscopy

In this work, FT-IR absorbance spectra of genomic DNAs isolated from four strains of Escherichia coli (A, B, C and D) and two strains of Salmonella (E and F), respectively, have been analyzed in both untreated and UV femtosecond laser pulses irradiated samples, to investigate their screening characteristics and their structural radiotolerance at 256 nm. Spectroscopic band assignments and structural aspects are discussed in the 600-1800 cm(-1) and 3000-4000 cm(-1) spectral ranges. DNA structural changes at the level of nucleic acids backbone, sugar, PO2- group, nitrogenous base ring and deoxycytidine (C2=O2) have been observed. Consequently, modifications in DNA conformations have been found. Bacterial strain dependent femtosecond laser pulses irradiation effects on DNA structure were observed. Based on FT-IR biomarkers novel early diagnosis and screening tools for routine use in health care and life sciences can be developed. Furthermore, cancer research could benefit from ultra-fast lasers technology. Besides, analyzing the interaction mechanisms of DNA with femtosecond laser pulses is highly appreciated for understanding the pathways of irradiation damaged DNA response in living cells.