Molecular Investigation Of Abortions Induced By Bunya Virus / Or Flavivirus Infections In Mazandaran Province, Northern Iran

The aim of this study was to identify border disease (BD) in aborted embryos or lambs with neurological disorders in different area of Mazandaran province, northern Iran. In this study, 75 aborted lambs and embryos were evaluated by Real time PCR using a commercial kit consisting of reverse transcript and polymerase enzyme that performs both reverse process and DNA polymerase activity. Brain and spinal cord samples were collected from each fetus by necropsy. In addition, in some cases, blood samples were collected randomly from sheep and aborted fetuses. After coagulation, the serum was isolated and stored at minus 20 degrees C until serological testing. We recorded only one case of infection with the border disease virus (BDV) in an embryo as well as PCR showed negative for brucella, listeria, campylobacter, and mycoplasma, and other viral agents, parasitic and fungal infectious agents. Therefore, this study suggests that aborted embryos and lambs with neurological problems need to be examined by molecular methods for pathogens such as Border virus.