Toll Like Receptor 3 Expression And Function On Peripheral Blood Mononuclear Cell In Pregnant Women With Hbv Infection

The aim of this study was to analyze the expression and function of TLR3 on PBMC in pregnant women with HBV infection. The expression of TLR3 mRNA on PBMC in 28 HBsAg positive pregnant women and 26 normal pregnant women was examined by semi‐quantitative RT‐PCR. The concentration of IL‐2, 6 in both serums of HBsAg positive and normal pregnant women were analyzed by ELISA. TLR3 mRNA was expressed at a higher level in HBsAg positive pregnant women(group A) than in normal pregnant women(group B). Group A were divided by their newborns having HBV intrauterine infection or not, the rate of TLR3Ct/β ‐actin Ct was not significant difference between the two groups. IL‐2 in group A serum was higher than group B,while IL‐6 was opposite to IL‐2. IL‐6 in serum of group A with their newborns having HBV intrauterine infection highly increased compared with not having HBV intrauterine infection women's. Correlation analysis showed that IL‐2 increased with the TLR3 mRNA increasing. The results demonstrated that TLR3 might predominantly promote Th1 immunological reaction against HBV infection.