Mir-486 Inhibits Gil1 Expression And Attenuates Epithelial Mesenchymal Transition And Invasion Of Prostate Cancer

Abnormal expression of glioma associated oncogene protein 1 (Gil1) is associated with various tumors. Studies have found that decreased miR-486 expression is associated with prostate cancer. Bioinformatics analysis revealed that miR-486 has a complementary binding site in the 3'-UTR region of Gli1 mRNA. This study investigated whether miR-486 plays a role in regulating Gli1 expression and affects epithelial mesenchymal transition (EMT) and invasion of prostate cancer cells. The dual luciferase reporter gene assay validated the targeted regulation between miR-486 and Gli1 qRT-PCR was used to measure the expression of miR-486 and Gli1 mRNA. The Gli1 protein expression was detected by western blot. PC3M cells were cultured in vitro and divided into two groups: miR-NC group and miR-486 mimic group. The expressions of Gli1, E-cadherin and N-cadherin were detected, and the cell invasion ability was detected by Transwell assay. There was a targeted regulatory relationship between miR-486 and Gli1 mRNA. Compared with RWPE-1 cells, miR-486 expression was significantly decreased and Gli1 expression was significantly increased in PC3 and PC3M cells, and both of them were increased in high metastatic PC3M cells. Transfection of miR-486 mimic significantly down-regulated the expression of Gli1 and N-cadherin in PC3M cells, which increased E-cadherin expression, inhibited EMT and decreased the invasive ability. The decreased expression of miR-486 plays a role in up regulating Gli1 expression, promotes EMT and invasion of prostate cancer cells. Increasing miR-486 expression can inhibit Gli1 expression and attenuate the EMT process and the invasion of prostate cancer cells.