Spectrofluorimetric Investigation Of Ages, Kyn, Nadh And Fadh In Dogs Suspected Of Having Diabetes Compared To Non-Diabetic Dogs

Diabetes mellitus (DM) is a common disorder in dogs, which is not monitored very effectively or diag-nosed on time. Detection of some biochemical abnor-malities in the diabetes pathogenesis ever since the be-ginning of disease suspicion remains an important goal concerning the early therapeutic intervention. This stu-dy aimed to use a rapid, economical and non-invasive method for the simultaneous evaluation of some fluo-rescent compounds whose mechanism of action is clo-sely related to the development of DM and its compli-cations. The spectrofluorimetric analysis was per -formed in 37 blood serum samples collected from dogs suspected of having diabetes and 11 blood serum sam-ples taken from dogs without suspicion of DM. It was attempted to establish associations and correlations between the following parameters: AGEs (advanced glycation end products), KYN (kynurenine), NADH (ni-cotinamide adenine dinucleotide), FAD (flavin adenine dinucleotide) and triglycerides, cholesterol, and glu-cose. In the case of dogs suspected of having diabetes compared to the control group was highlighted a signi-ficant increase in the contributions of all analysed fluo-rophore compounds. Also, a significant correlation was established between the contribution of NADH and the blood glucose level, the age of the investigated dogs, respectively. The results show in dogs, as in humans, the importance of monitoring these serum fluorophore parameters ever since the beginning of diabetes suspi-cion in order to avoid complications during the progre-ssion of diabetes and respectively to find disease-rela-ted new pathogenetic mechanisms.