The Novel Target Of Liver Cancer: Microrna-4324 Regulates Cell Proliferation And Migration Via Targeting Neuraminidase 3

Background: MicroRNA-4324 has been reported to regulate various biological malignant cancer. Nonetheless, the expression and molecular mechanism of miR-4324 in liver cancer remain rarely known. This study aimed to investigate the effect miR-4324 on the proliferation, invasion and migration of hepatoma cells. Methods: The mRNA level of miR-4324 was assessed in four hepatoma cell lines (HepG2, Huh7, MHCC97, HB611) and human embryonic liver cell, HHL5. MiR-4324 was overexpressed in hepatoma cells. Subsequently, the effects of miR-4324 on cell proliferation, migration and invasion and the underlying molecular mechanisms were detected. Results: Our data indicated that miR-4324 was down-regulation in hepatoma cell lines compared with HHL5. Overexpression of miR-4324 inhibits cellular proliferation, colony-formation, migration and invasion abilities of hepatoma cells. However, the biological effects of miR-4324 overexpression on hepatoma cells were reversed after overexpressing NEU3. Conclusions: Our findings concluded that miR-4324 inhibits biological functions of hepatoma cells by targeting NEU3 and it might be a potential target for the treatment of liver cancer.