Long Non-Coding Rna Small Nucleolar Rna Host Gene 1 And Microrna-100-3p Expression In Endometrial Carcinoma And Its Effect On The Proliferation And Apoptosis Of Ishikawa Cells
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING(2021)
Abstract
The aim of this study was to explore LncRNA SNHG1 and miRNA-100-3p expression in endometrial carcinoma and its effect on the proliferation and apoptosis of Ishikawa cells. A qRT-PCR assay was conducted to determine SNHG1 and miRNA-100-3p expression in endometrial cancer tissues and paracancerous tissues. Human endometrial cancer Ishikawa cells were cultured in vitro. Si-NC, si-SNHG1, si-SNHG1, anti-miRNA-NC, and si-SNHG1 were transfected into Ishikawa cells with anti-miRNA-519b-3p. A qRT-PCR assay was performed to determine SNHG1 and miRNA-100-3p expression, and the CCK-8 method was used to determine cell proliferation. Flow cytometry was conducted to determine cell cycle and apoptosis rate and a dual luciferase reporter experiment was carried out to test the targeting association between SNHG1 and miRNA-100-3p. Cleaved Caspase-3, CHOP, and ATF4 expression were determined with the Western Blot method. SNHG1 expression level and miRNA-519b-3p expression level were much higher and much lower, respectively, in endometrial cancer tissues than in paracancerous tissues (P < 0.05). Transfection of si-SNHG1 can greatly attenuate cell viability and S cell ratio (P < 0.05), and increase G0/G1 cell ratio, apoptosis rate, Cleaved Caspase-3, CHOP, and ATF4 protein level (P < 0.05) compared to the si-NC group. Furthermore, the double luciferase reporter experiment confirmed that SNHG1 can competitively combine with miRNA-100-3p. Also, co-transfection of si-SNHG1 and anti-miRNA100-3p could significantly increase cell viability and S cell ratio (P < 0.05), and decrease G0/G1 cell ratio and apoptosis rate, and Cleaved Caspase-3, CHOP, and ATF4 protein levels compared to si-SNHG1 + anti-miRNA-NC (P < 0.05). Interfering with SNHG1 could inhibit the proliferation of Ishikawa cells and promote apoptosis by upregulating miRNA-100-3p expression.
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Key words
LncRNA SNHG1, miRNA-100-3p, Endometrial Cancer, Ishikawa Cells, Proliferation, Apoptosis
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