Efficient biocatalysis of trillin through recombinant enzyme hydrolysis for clean diosgenin production
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION(2021)
Abstract
Diosgenin has been widely used as a precursor in the pharmaceutical industry. The conventional diosgenin production method, direct acid hydrolysis, can cause severe environmental pollution and has low production efficiency. In this study, the beta-glucosidase FBG1 was successfully over-expressed in Pichia pastoris. The engineered yeast was grown in 5- and 50-L bioreactors to a high-cell-density, and the resulting recombinant FBGI without purification was directly used for trillin biocatalysis. A novel enzymatic biocatalysis method was established through efficient biocatalysis of trillin by recombinant FBGI and a practical diosgenin purification process. Diosgenin production reached similar to 1.7 mg/mL under optimized biocatalysis conditions with a corresponding similar to 94 % trillin conversion. The production process was further scaled up from 2 mL to 20, 200, and 2000 mL working volumes, producing equivalent efficiency. This recombinant enzyme biocatalysis process resulted in similar to 680 mg diosgenin when 1 g trillin was processed, together with similar to 90 % environmental impact elimination and similar to 36 % cost reduction. Enzymatic biocatalysis can convert trillin into a high-value-added pharmaceutical precursor of diosgenin with high eco-efficiency, cost-effectiveness, and sustainability is presented here for the first time and is a promising method for future applications in industrial processes. (C) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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Key words
Trillin,Recombinant beta-glucosidase,Enzymatic biocatalysis,Diosgenin,Pichia pastoris,High-value-added product
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