Engineering A Whole Rat Liver Scaffold
DOKLADY NATSIONALNOI AKADEMII NAUK BELARUSI(2021)
Abstract
Engineering a three-dimensional scaffold opens up great prospects for creation of manufacturing biological artificial organs. The article presents a method of perfusion decellularization of a rat liver, with the main problems and options for their solution being analyzed. Perfusion of a donor liver with 0.1 % a sodium dodecyl sulfate (SDS) solution allows obtaining a high-quality cell-free matrix characterized by preserved hepatic architectonics, patent vascular bed, residual DNA of less than 1 %, no signs of collagen fibers destruction and tissue edema. The obtained scaffold can be used for recellularization by allogeneic cell cultures when creating volumetric tissue-engineered designs.
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Key words
decellularization, liver scaffold, residual DNA, cytotoxicity, perfusion
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