Feasibility Of Drug-Eluting Biodegradable Stent For Ureteral Stricture Treatment In An Animal Model

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Ureter (including Pyeloplasty) and Bladder Reconstruction (including fistula), Augmentation, Substitution, Diversion II (MP53)1 Sep 2021MP53-19 FEASIBILITY OF DRUG-ELUTING BIODEGRADABLE STENT FOR URETERAL STRICTURE TREATMENT IN AN ANIMAL MODEL Wai-Nga Chan, Dong-Ru Ho, Chih-Shou Chen, Wei-Yu Lin, Yun-Ching Huang, Jian-Hui Lin, Kuo-Tsai Huang, and Yu-Liang Liu Wai-Nga ChanWai-Nga Chan More articles by this author , Dong-Ru HoDong-Ru Ho More articles by this author , Chih-Shou ChenChih-Shou Chen More articles by this author , Wei-Yu LinWei-Yu Lin More articles by this author , Yun-Ching HuangYun-Ching Huang More articles by this author , Jian-Hui LinJian-Hui Lin More articles by this author , Kuo-Tsai HuangKuo-Tsai Huang More articles by this author , and Yu-Liang LiuYu-Liang Liu More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002083.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Fibrosis plays an important role in ureteral stricture. The aim of the present study is to evaluate the effectiveness and histopathological sequela of a rapamycin drug-eluting biodegradable ureteral stent for treatment of ureteral stricture using a rabbit model. METHODS: Dose dependent effect of rapamycin on ureter component cell lines are tested. Fibrogenic signaling proteins, including a-SMA and Smad was evaluated with or without TGF-b co-treatment. Upper ureter fibrosis was induced by open surgery with thermal injury male rabbits. Biodegradable stents, with or without rapamycin drug eluting, were inserted. Gross anatomy changes, along with collagen deposition were evaluated with Masson’s trichrome stain. Smad, a-SMA, and SNAI 1 expressions were checked by immunohistochemistry and Western blotting 4 weeks after the operation. RESULTS: COL3A1 is the major collagen subtype depositing in rabbit ureteral healing process. Its deposition decreased in ureter thermal injury groups with or without drug-eluting stents. Rapamycin-eluting biodegradable stent can affect TGF-b and EMT signaling pathway by significantly modifying Smad and SNAI 1 expression, respectively. Hydronephrosis was severe in thermally injured ureter without stents. Once stents are in place and effective, only pelvicectasis would be found. However, the gross and histopathology of ureter remained similar between stented groups with or without drug-eluting, but they are significantly different compared to sham operated controls. CONCLUSIONS: Intralumenal administration of rapamycin by implanting bioresorbable scaffold may modify associated signaling pathway. mTOR inhibitor, applying with other effective chemicals, may be a promising way to prevent ureteral stricture after thermal injury. Source of Funding: This work was supported by grants from the Chang Gung Medical Foundation, Grant Number CMRPG6C0313, CMRPG6G0381, and CMRPG6H0551 © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e946-e947 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Wai-Nga Chan More articles by this author Dong-Ru Ho More articles by this author Chih-Shou Chen More articles by this author Wei-Yu Lin More articles by this author Yun-Ching Huang More articles by this author Jian-Hui Lin More articles by this author Kuo-Tsai Huang More articles by this author Yu-Liang Liu More articles by this author Expand All Advertisement Loading ...