PD52-04 STIMULATED RAMAN SPECTROSCOPY AS A METHOD TO DETERMINE THE ADEQUACY OF RENAL MASS BIOPSY

You have accessJournal of UrologyKidney Cancer: Epidemiology & Evaluation/Staging/Surveillance II (PD52)1 Sep 2021PD52-04 STIMULATED RAMAN SPECTROSCOPY AS A METHOD TO DETERMINE THE ADEQUACY OF RENAL MASS BIOPSY Miles P Mannas, Derek Jones, Fang-Ming Deng, Daniel Orringer, and Samir S Taneja Miles P MannasMiles P Mannas More articles by this author , Derek JonesDerek Jones More articles by this author , Fang-Ming DengFang-Ming Deng More articles by this author , Daniel OrringerDaniel Orringer More articles by this author , and Samir S TanejaSamir S Taneja More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002079.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Renal biopsy requires adequate tissue sampling to aid in the investigation of renal masses. The contemporary rate of non-diagnostic renal biopsy ranges from 15-18%, though may be as high as 42% in challenging cases. Simulated Raman Histology (SRH) is a novel microscopic technique which has created the possibility for rapid, label-free, and high-resolution images of unprocessed tissue which may be viewed on standard radiology viewing platforms. The application of SRH to renal biopsy may provide the benefits of pathologic hematoxylin and eosin (H&E) evaluation during the procedure, thereby reducing non-diagnostic results. METHODS: An 18-gauge core needle biopsy was taken of renal masses from a radical or partial nephrectomy specimens. Histologic images of the fresh, unstained biopsy samples were obtained using a Stimulated Raman Spectroscopy microscope using two Raman shifts: 2845cm-1 and 2930cm-1. These Stimulated Raman Spectroscopic images were then processed to create SRH, which is reminiscent of H&E, and viewed by a genitourinary pathologist. RESULTS: The SRH microscope took 7 minutes to produce a high-quality image of the renal biopsies, figure 1 and 2. The images were interpreted by a genitourinary pathologist and areas of tumor were easily identified as lesional. Figure 1a shows SRH of medullary renal carcinoma, while figure 1b shows representative biopsy SRH for clear cell renal cell carcinoma. CONCLUSIONS: SRH produces high quality images that can be rapidly produced and easily interpreted to determine renal mass biopsy adequacy. Procedural application has promise to decrease the rate of renal mass non-diagnostic biopsies. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e912-e912 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Miles P Mannas More articles by this author Derek Jones More articles by this author Fang-Ming Deng More articles by this author Daniel Orringer More articles by this author Samir S Taneja More articles by this author Expand All Advertisement Loading ...