Preparation of Antioxidant and Antibacterial Chitosan Film from Periplaneta americana

Simple Summary The American cockroach (Periplaneta americana) is a kind of insect distributed worldwide. Commonly, it is considered as a pest. However, nowadays, it has been developed as a potential resource of protein, lipid, and antibacterial peptide. Besides, it also contains chitin, which could be used to produce chitosan by deacetylation. Chitosan is a valuable biomaterial containing amino groups, and has been applied in various fields. However, the researches focusing on the applications of P. americana chitosan are rare, which might hinder the exploration of the value of P. americana. In this paper, we prepared and characterized the chitosan film from P. americana. The performances relating to food packaging of the obtained film were also examined. As the results showed, P. americana chitosan film could resist UV light effectively. It could also keep scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals in 8 h, proving its ability of antioxidant. In addition, it exhibited antibacterial activity by resisting the growth of Serratia marcescens and Escherichia coli. The results showed that P. americana chitosan film could work as a potential food packaging material, which implicated the value of P. americana chitosan and provided a new clue for the exploration of the value of more insects, especially pests. Among different insects, the American cockroach (Periplaneta americana) has been bred in industrial scale successfully as a potential resource of protein, lipid, and antibacterial peptide. However, the application of its chitosan has not been studied widely, which has hindered the sufficient utilization of P. americana. In this paper, the chitosan from P. americana was separated, characterized, and processed into film (PaCSF) to examine its potential of being applied in food packaging. As the results of different characterizations showed, PaCSF was similar to shrimp chitosan film (SCSF). However, concerning the performances relating to food packaging, the two chitosan films were different. PaCSF contained more water (42.82%) than SCSF did, resulting in its larger thickness (0.08 mm). PaCSF could resist UV light more effectively than SCSF did. Concerning antioxidant activity, the DPPH radical scavenging ability of PaCSF increased linearly with time passing, reaching 72.46% after 8 h, which was better than that of SCSF. The antibacterial activity assay exhibited that PaCSF resisted the growth of Serratia marcescens and Escherichia coli more effectively than SCSF did. The results implied that P. americana chitosan could be a potential raw material for food packaging, providing a new way to develop P. americana.