Targeted Mutation Analysis Of Acute Myeloid Leukemia At Diagnosis And Relapse Shows Different Clonal Evolution Patterns Involving Different Functional Classes Of Genes

AML relapse is often associated with a clonal evolution at the cytogenetic and molecular level and therefore represents a challenge for the treatment. Targeted sequencing is now usually done at diagnosis in AML, as only a small core group of genes is frequently mutated in AML and MDS. This approach, contrary to Whole Genome Sequencing, is cheaper and allows the detection of variant allele fractions as low as 2% with a rapid turnover and high sequencing coverage and depths.