Cloning And Function Research Of Sheepgrass (Leymus Chinensis) Genes

Sheepgrass is a dominant, perennial forage crop that has extensive plasticity in adapting to various harsh environments, such as high salinity, drought, and cold stresses. Based on the data from 454 high-throughput sequencing (GS FLX) exposure to abiotic stress, a series of stress-responsive genes from sheepgrass have been identified and characterized, including LcDREB2, LcDREB3a, LcDREB21, LcSAIN1, LcSAIN2, LcMYB1, LcWRKY5, LcFIN1, LcP5CSs, LcSAMDCs, LcSAP, LcPIP, and so on; these genes play different role in abiotic stresses of salinity, drought, and cold. Otherwise, the sucrose synthesis and transport-related genes were also isolated and identified, i.e., LcSUT1, etc. The research on the gene function of sheepgrass provide understanding for the mechanism of sheepgrass tolerance and adaption to adverse environments, and these genes are potential gene resource for improving abiotic tolerance and sucrose transport in sheepgrass and other crops.