Exploring Brain Genoarchitecture By Single And Double Chromogenic In Situ Hybridization (Ish) And Immunohistochemistry (Ihc) On Cryostat, Paraffin, Or Floating Sections

Jose Luis Ferran,Abdelmalik Ayad,Paloma Merchan,Nicanor Morales-Delgado,Luisa Sanchez-Arrones,Antonia Alonso,Juan Eugenio Sandoval,Sylvia Marianne Bardet,Ruben Corral-San-Miguel,Luis Oscar Sanchez-Guardado,Matias Hidalgo-Sanchez,Margaret Martinez-De-La-Torre,Luis Puelles

IN SITU HYBRIDIZATION METHODS（2015）

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摘要

This chapter is devoted to explain how to handle with brain specimens to get cryostat, paraffin, or floating vibratome sections and the way they are processed during ISH and IHC. The procedure to be selected is related to the study aim, general and rapid mapping, or detailed and high resolution analysis. We likewise place emphasis on how to block (embed) and prepare the tissue blocks for sectioning, insuring that optimal section planes are obtained.

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关键词

In situ hybridization, Gene expression patterns, RNA detection, Riboprobes, Labeled riboprobes, Digoxigenin, Fluorescein, FITC, RNA probes, Paraffin sections, Floating sections, Cryostat sections, Microtome, Cryostat, Vibratome

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