Exploring Brain Genoarchitecture By Single And Double Chromogenic In Situ Hybridization (Ish) And Immunohistochemistry (Ihc) In Whole-Mount Embryos

Jose Luis Ferran,Abdelmalik Ayad,Paloma Merchan,Nicanor Morales-Delgado,Luisa Sanchez-Arrones,Antonia Alonso,Juan Eugenio Sandoval,Sylvia Marianne Bardet,Ruben Corral-San-Miguel,Luis Oscar Sanchez-Guardado,Matias Hidalgo-Sanchez,Margaret Martinez-De-La-Torre,Luis Puelles

IN SITU HYBRIDIZATION METHODS（2015）

Cited 11|Views4

No score

Abstract

This chapter and 5 detail various in situ hybridization protocols perfected over time in two laboratories with widespread experience in studying brain development and comparative neuroanatomy in amphibian, avian, and mammalian animal models. The aims of our research have frequently revolved on studying high-quality maps of gene expression-genoarchitecture-in the developing or adult brain. Apart of the protocols themselves, in this chapter, detailed advice is given as well about ways to treat the brain specimens to insure little damage and good histological quality during whole-mount ISH and IHC.

Translated text

Key words

In situ hybridization, Gene expression patterns, RNA detection, Riboprobes, Labeled riboprobes, Digoxigenin, Fluorescein, FITC, RNA probes, Whole mount

AI Read Science

Must-Reading Tree

Example

Generate MRT to find the research sequence of this paper

Chat Paper

Summary is being generated by the instructions you defined