A rapid and simplified methodology for the extraction and quantification of allicin in garlic

•A simplified and validated method for allicin analysis in garlic was developed.•An optimal solution and sample preparation for allicin extraction was established.•Highest allicin was extracted from fresh garlic, followed by freeze-dried cloves.•The stability of allicin extract under different storage conditions was determined.•Allicin concentration was accurately determined in eight garlic varieties.