Recombinant Human Acid Fibroblast Growth Factor Accelerates Skin Wound Healing In Diabetic Rats Through Promoting Fibroblast Proliferation And Angiogenesis

The aim of the present study is to investigate the effect and mechanism of recombinant human acidic fibroblast growth factor (rhaFGF) on skin wound healing of diabetic rats. SD rats were randomly divided into three groups: control, diabetes and rhaFGF groups. Skin wound tissues of rats in the three groups were respectively arranged on days 3 and 7 after establishment of skin wound of diabetic rats. Immunohistochemistry analyses were performed to evaluate the effect of rhaFGF on expression levels of PCNA and CD34 in diabetic rats. Real-time PCR and Western blot assays were used to examine the expression levels of Bcl-2, Bax, TGF-beta, CXCL-1, CXCL-2, p-ERK1/2 and p-STAT3 in wound tissues. The results showed that rhaFGF could significantly increased expression levels of PCNA, CD34, TGF-beta, CXCL-1, CXCL-2, p-ERK1/2, p-STAT3 and the ratio of Bcl-2/Bax in wound tissues. The preliminary mechanism that rhaFGF accelerates wound healing of diabetic rats is possibly associated with the promotion of fibroblasts proliferation and angiogenesis through ERK1/2 and STAT3 pathways.