Effects Of Nano-Selenium On Blood Biochemistry, Liver Antioxidant Activity And Gpx-1 Mrna Expression In Rabbits

The experiment was conducted to compare the effect of nano-selenium and sodium selenite on blood biochemistry, liver antioxidant activity and GPx-1 mRNA expression in rabbit. Twenty-four 10-week-old New Zealand rabbits, female and male in half, were randomly divided into three groups. Rabbits in Group 1 were fed a basal diet as control, while rabbits in Groups 2 and 3 were fed the basal diet supplemented with 0.3 mg/kg selenium as sodium selenite or nano-selenium, respectively. The experiment lasted for 42 days. Blood biochemical values, glutathione peroxidase (GPx) activity, total antioxidant capacity (T-AOC), malondialdehyde (MDA) content and glutathione peroxidase. (GPx-1) mRNA expression in liver were determined at experiment termination. The results showed that blood biochemistry of rabbits, including concentrations of cholesterin, triglyceride, total protein, glucose, alanine amino transferase and aspartate amino transferase were not significantly affected by supplementation of nano-selenium or sodium selenite. Liver GPx and T-AOC activities in nano-selenium group and sodium selenite group were significantly higher or higher than those in control group (P<0.01 or P<0.05), respectively. Liver MDA contents in nano-selenium group and sodium selenite group were significantly lower than those in control group (P<0.01), while liver MDA contents in nano-selenium group were lower than those in sodium selenite group(P<0.05).Liver mRNA expression of GPx-1 was approx 195% higher in nano-selenium group and approx 154% higher in sodium selenite group than that in control group. While, there was no significant difference in the increase of liver GPx-1 mRNA expression between sodium selenite and nano-selenium groups (P>0.05). In conclusion, nano-selenium supplementation could significantly enhance liver antioxidant activity and GPx-1 mRNA expression in rabbits, it was more available than sodium selenite in increasing liver antioxidant activity in rabbits.