Expression And Effects Of Mir-26a-5p In Regulating The Pten/Akt Pathway In Renal Tissues And Cells Of Diabetic Mice

This study aims to investigate the expression and effects of miR-26a-5p in regulating the PTEN/AKT pathway in renal tissues and cells of diabetic mice. A total of 40 mice were stochastically divided into a normal control group (n=10) and a diabetes model group (DM group, n=30). Normal diet was adopted in the normal group, while high fat diet was adopted in the DM group. The expression changes of miR-26a-5p and PTEN/AKT in the murine renal tissues were dynamically measured by real-time fluorescence quantitative PCR. The histopathological changes of the renal tissues were observed with H&E under colored light microscope. Through detecting miR-26a-5p and PTEN/AKT expression in mouse tissues and renal cells and transfecting the them into cells by up-regulating or down-regulatingtheir expression; the levels of apoptosis-related proteins Caspase 3, Caspase 9 and Bax/Bcl-2 were analyzed, and cell proliferation, invasion and apoptosis mechanisms were studied. miR-26a-5p was less expressed in the DM group but had more highly expressed PTEN/AKT. miR-26a-5p down-regulation or PTEN/AKT up-regulation could notably promote malignant proliferation and invasion in diabetic mice, inhibit cell apoptosis levels, and down-regulate the expression and protein levels of Caspase 3, Caspase 9, Bax/Bcl-2. Dual luciferase reporter confirmed that miR-26a-5p had targeted relationship with PTEN. When up-regulating miR-26a-5p and down-regulating PTEN/ AKT at the same time, the malignant progression of cells was not different from miR-NC transfected cells with unrelated sequences. miR-26a-5p promotes cell survival in diabetic mice by down-regulating PTEN/AKT, which may become a new direction for diagnosis and gene therapy of diabetes.