Vitamin D3 Intervention Can Improve Inflammatory Response And Immune Function In Colorectal Cancer Mouse Model

To investigate the effect of vitamin D3 (VD3) on inflammatory factors and immune function of colorectal cancer (CRC) mice model. 125 SPF BALB/c mice were purchased and divided into model group, VD3 low dose group, VD3 medium dose group, VD3 high dose group and normal group on average. Tumorigenesis was induced in mice except the normal group by human CRC cell line HCT116. After administration for 4 weeks, tumor volume, thymus index, spleen index, survival time, cell cycle and apoptosis rate of CRC mice were recorded, and the effect of VD3 on inflammatory factors and immune indexes of CRC mice was detected. The intake of VD3 contributed to the reduction of tumor size, the improvement of thymus and spleen indexes, the extension of survival time, the stagnation of G0/G1 phase, the increase of apoptosis rate, the reduction of concentration of proinflammatory factors high sensitivity C-reactive protein (Hs-CRP), interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), the increase of concentration of anti-inflammatory factor interleukin-4 (IL-4), the increase of phosphorylation level of inflammatory proteins SMAD2/3 and SMAD7, the decrease of transforming growth factor-beta 1 (TGF-beta 1) level, the increase of cellular immune index CD4(+), CD4(+)/CD8(+) levels, the decrease of CD8(+) level and the increase of humoral immune index antibody-producing cells and hemolysin secretion in CRC mice. VD3 intervention can improve inflammatory response and immune function in CRC mouse model.