Modified Method Of Fuzzy Recognition Of Proteins In Electrophoresis In Population Genetics

We propose the development of a previously created method for fuzzy recognition of proteins by gel electrophoresis of earthworm tissues in population genetics. In case when several proteins have close masses, their traces on the electrophoregram intersect. The visually determined boundaries of the fuzzy membership function carrier are unjustifiably narrowed, as can be seen in the dependence of the boundaries on the mass of the protein. Therefore, it is necessary to clarify the coordinates of the carrier of the fuzzy membership function for each of them. The methodology developed by us involves the construction of an experimental membership function on a narrowed carrier, the approximation of it by selected curve (Gauss or Bezier of fifth order) and, then, the adjustment of the boundaries of the carrier by extrapolating the approximants to such boundaries where the membership function reaches a predetermined level. The effectiveness of the application of the technique in studying the effect of laser radiation on changing the genotype of the Eisenia foetida worm population is shown.