Biodegradation Of Diphenyl Ortho-Phthalate Dpp By Pseudomonas Sp.Bet-7 Isolated From Active Sludge

Diphenyl ortho-phthalate (DPP), as a prior pollutant for control, has been widely detected in environment. One bacterial strain was isolated from activated sludge using Diphenyl ortho-phthalate as the sole source of carbon and energy. According to the phylogeny of 16S rDNA sequence, the bacterial strain BET-7 was identified as Pseudomonas sp. Biodegradation of DPP by Pseudomonas sp. BET-7 was investigated. Results showed that the optimum pH and temperature for DPP degradation by BET-7 were 8.0 and 15 degrees C. The concentration of degradation ranged from 100 mg. L-1-400 mg. L-1. The strain exhibited higher degradation efficiencies and growth rates in alkalinity than in acidity. The optimum temperature was lower than 20 degrees C. The degradation rate decreased when temperature went up. The strain could degrade approximately 90% of 400 mg/L DPP after 60 h of cultivation. The results suggest that BET-7 may represent a promising application for DPP bioremediation.