Differences In Platelet Proteins Between Acute Lymphoblastic Leukemia And Healthy Children

Background: Acute lymphoblastic leukemia (ALL) is the most common malignant cancer in the childhood. The researches about the changes of platelet function in ALL are still rare, and the specific mechanism is still unknown. Due to the limited detection technology, the functions of platelet in ALL are poorly understood. Purpose: To investigate the expression difference of platelet proteins between ALL and healthy children. Methods: Twenty-seven ALL children (ALL group), 25 ALL children who obtained complete remission after remission-induction therapy (ALL-CR1 group) and 27 healthy children (control group) were enrolled in this study. The surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technique was used to obtain and analyze the whole platelet protein spectrum of each group. Results: Compared with control group, ALL group had 9 protein peaks which had the stable expression differences (P < 0.05), the mass/charge ratio (m/z) were 2496.9, 4287.9, 7881.2, 4091.3, 3149.9, 2365.1, 9414.1, 5252.3 and 2280.7, respectively. There were 6 protein peaks with the stable expression differences between the ALL group and the ALL-CR1 group (P < 0.05), and the m/z were 2496.9, 4287.9, 9414.1, 7881.2, 3149.9 and 2280.7, respectively. The differentially expressed protein peaks were analyzed as endothelin-1, big endothelin-1, PF4, thrombin light chain, pituitary adenylate cyclase activating polypeptide 27, fibrinogen beta chain and 3 unknown proteins. Conclusion: There are significant differences in platelet proteome between ALL children and healthy children, and the ALL children have the platelet function abnormalities, such as the obstacle of cell signal transduction, aggregation, and abnormalities of activation and coagulation.