Cell-Oxidative Damage To Hek293t Cells Induced By Combination Of Cdcl2 And Nano-Tio2, Cdcl2 And Edta

[Objective] To investigate the cell-oxidative damage to Human Embryo Kidney 293t (HEK293t) cells induced by CdCl2 and EDTA. [Methods] RT-PCR technique was used to detect the expressions of gene HO-1 and OGG1. Activities of antioxidases and concentrations of ROS were also detected to evaluate the conjoined oxidative damage of CdCl2 and EDTA to HEK293t cells. [Results] The results of HO-1 gene expressions showed that the observed interaction between of CdCl2 and EDTA were additive at low concentations; at high concentrations, the observed interaction was antagonism. The results of OGG1 gene expressions showed that the observed interaction were additive at high CdCl2 proportions; at low CdCl2 proportions, the observed interaction was synergism when concentration was low and antagonism when concentration was high. The results of activities of SOD, CAT and concentrations of MDA showed that the observed interactions were additive at low concentrations and antagonism at high concentrations. The results of concentrations of ROS showed that the observed interaction was antagonism. [Conclusion] The interaction between CdCl2 and EDTA was antagonism. Influences of concentration and ratio to these indexes were differently meant that sensitivities of all these indexes were various.