Peculiarities Of Bacillus-Subtilis Competent Cells And Protoplasts Transformation By Dnas Of Integrative Plasmids

The effect of structural peculiarities of DNAs from integrative plasmids on the transformation activity was studied. Monomeric forms of the plasmids can only transform B. subtilis competent cells, when plasmid selective marker is inserted into chromosomal fragment within the plasmid. Polymeric forms are needed for efficient transformation. Both single- and double-stranded DNAs of integrative plasmids transform no B. subtilis protoplasts, this being irrespective of plasmid structure.